Standards & References

European standards and benchmarks

NF EN 1040

European norm

Determination of basic bactericidal activity – phase 1

  • Pseudomonas aeruginosa – Staphylococcus aureus
  • Microbial reduction >105
  • Contact time: 1, 5, 15, 30, 45 or 60 minutes
  • 20°C

NF EN 1275

European norm

Determination of basic fungicidal activity

  • Candida albicans – Aspergillus niger
  • Microbial reduction >104
  • Contact time: 5, 15, 30 or 60 minutes
  • 20°C

NF EN 1276

European norm

Determination of the bactericidal activity of disinfectants used in the food industry, industrial, domestic and community sectors – phase 2, step 1

  • Pseudomonas aeruginosa – Escherichia coli – Staphylococcus hirae and other optional additional strains
  • Microbial reduction >105
  • Contact time: 5 minutes and other additional times optional
  • 20°C and other optional additional temperatures
  • Dirt conditions: 3 g/L bovine albumin
  • Cleanliness conditions: 0.3 g/L of bovine albumin

European standards and benchmarks

NF T 72-150 or T 72-151

French standard

Determination of bactericidal activity spectrum 4 or spectrum 5

  • Pseudomonas aeruginosa – Escherichia coli – Staphylococcus aureus – Enterococcus hirae (Mycobacterium Smegmatis)
  • Microbial reduction >105
  • Contact time: 5 minutes
  • 20°C

NF T 72-170 or T 72-171

French standard

Determination of bactericidal activity spectrum 4 or 5 in the presence of interfering substances

  • Pseudomonas aeruginosa – Escherichia coli – Staphylococcus aureus – Enterococcus hirae (Mycobacterium Smegmatis)
  • Microbial reduction >105
  • Contact time: 5 minutes
  • 20°C
  • Hard water: 60° French – Proteins: albumin 1% + yeast extract 1% – Buffer solutions pH 5 and pH 9 – Dirt conditions: hard water 30° French + albumin 0.3%

NF T 72-180

French standard

Determination of virucidal activity against vertebrate viruses

  • Enterovirus Polio I, Sabin strain – Human andenovirus type 5 – Vaccinia orthopoxvirus
  • Viral reduction >104
  • Contact time: 15, 30 and 60 minutes
  • 20°C

NF T 72-181

French standard

Determination of activity towards bacteriophages

  • Bacteriophage T 2 – Bacteriophage MS 2 – Bacteriophage OX 174 – Bacteriophage n°66
  • Viral reduction >104
  • Contact time: 15 minutes
  • 20°C

NF T 72-190

French standard

Determination of bactericidal, fungicidal and sporicidal activity – so-called germ carrier method

  • Strains referenced in standards, contact time and temperature not fixed: NF T 72-150 or 151 Microbial reduction >105
  • NF T 72-200 or 201 Microbial reduction >104
  • NF T 72-230 or 231 Microbial reduction >103

NF T 72-300 or T 72-301

French standard

Determination of the effectiveness of products on various microorganisms under practical conditions of use

  • Bacteria reduction >105
  • Reduction of yeasts and molds >104
  • Reduction of bacterial spores >103
  • Contact time: not fixed
  • Temperature not fixed
  • Interfering substances: water 30°HF or variable depending on the conditions of use

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